interaction of doxorubicin with dna-hmg1 complex

in this study, the interaction of the anthracycline antibiotic doxorubicin with dna-hmg 1 complex was investigated employing uv/vis spectroscopy, thermal denaturation and dna cellulose chromatography techniques. the results indicated that the binding of doxorubicin to the protein reduces its tm in a dose dependent manner. although doxorubicin protects free dna against thermal denaturation in the presence of hmg 1, thermal melts represent biphasic profile corresponding to dna- drug, hmg1- drug and free components. interaction of antibiotic with dna - hmgl complex using dna cellulose columns also implies that the drug binds strongly to singlestranded dna-hmg 1 complex rather than to double- stranded dna-hmg 1 columns. the results suggest destabilization of hmgl protein by drug action thereby preventing dna-hmg 1 complex formation